The nitroreductase system of inducible targeted ablation facilitates cell-specific regenerative studies in zebrafish
نویسندگان
چکیده
منابع مشابه
Targeted ablation of beta cells in the embryonic zebrafish pancreas using E. coli nitroreductase
In order to generate a zebrafish model of beta cell regeneration, we have expressed an Escherichia coli gene called nfsB in the beta cells of embryonic zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme, which can convert prodrugs such as metronidazole (Met) to cytotoxins. By fusing nfsB to mCherry, we can simultaneously render beta cells susceptible to prodrug and visualize M...
متن کاملTargeted Pth4-expressing cell ablation impairs skeletal mineralization in zebrafish
Skeletal development and mineralization are essential processes driven by the coordinated action of neural signals, circulating molecules and local factors. Our previous studies revealed that the novel neuropeptide Pth4, synthesized by hypothalamic cells, was involved in bone metabolism via phosphate regulation in adult zebrafish. Here, we investigate the role of pth4 during skeletal developmen...
متن کاملConditional targeted cell ablation in zebrafish: a new tool for regeneration studies.
Conditional targeted cell ablation in zebrafish would greatly expand the utility of this genetic model system in developmental and regeneration studies, given its extensive regenerative capabilities. Here, we show that, by combining chemical and genetic tools, one can ablate cells in a temporal- and spatial-specific manner in zebrafish larvae. For this purpose, we used the bacterial Nitroreduct...
متن کاملstrategies applied in translation of culture-specific items in translation of the i. r. i. constitution &the civil code (from persian into english(
این تحقیق به بررسی استراتژیهای بکاررفته توسط مترجمین مختلف در ترجمه واژه های فرهنگی خاص در ترجمه فارسی به انگلیسی قانون مدنی و قانون اساسی جمهوری اسلامی ایران میپردازد.این تحقیق بر اساس نظریه پیتر نیومارک صرت گرفته است. در این تحفیق واژه های فرهنگی خاص اقتباس شده و پس از مقایسه با متن مبدا به بررسی انها پرداخته می شود.در قسمت ضمائم تحقیق واژه نامه ای برای اشنایی خوانندگان با لغات و اصطلاحات حقو...
A zebrafish model of conditional targeted podocyte ablation and regeneration
Podocytes are specialized cells that contribute critically to the normal structure and function of the glomerular filtration barrier. Their depletion plays an important role in the pathogenesis of glomerulosclerosis. Here, we report generation of a genetic model of conditional podocyte ablation and regeneration in zebrafish using a bacterial nitroreductase strategy to convert a prodrug, metroni...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Methods
سال: 2013
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2013.03.017